Hotspot

trnQ-UUG-psbK

Genome · 7037-7362 · 326 bp

Back to Achatocarpus

Pi 0.00307

MarkerSeek score 54.80

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: Genome
length_bp: 326
variable_sites: 1
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CATATGCTCTGGGACGGAAG	AGGGCAGAATTAAGACAGATCA	55.0	40.9	57.8	56.9	447 bp	1.0	53.9
trnQ-UUG-psbK_p2	GACCAAAACCCGATGCCTTA	AGGGCAGAATTAAGACAGATCA	50.0	40.9	58.2	56.9	405 bp	1.0	53.9
trnQ-UUG-psbK_p3	TACATATGCTCTGGGACGGA	AGGGCAGAATTAAGACAGATCA	50.0	40.9	57.6	56.9	449 bp	1.0	52.2
trnQ-UUG-psbK_p4	AAAACCCGATGCCTTACCAC	AGGGCAGAATTAAGACAGATCA	50.0	40.9	58.5	56.9	401 bp	1.0	51.6
trnQ-UUG-psbK_p5	ACCAAAACCCGATGCCTTAC	AGGGCAGAATTAAGACAGATCA	50.0	40.9	58.5	56.9	404 bp	1.0	51.6