Hotspot

trnK-UUU-rps16

Genome · 4418-5164 · 747 bp

Back to Achatocarpus

Pi 0
MarkerSeek score 58.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
Genome
length_bp
747
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTAGCCGCACTTAAAAGCC AAGAGGGCAGAGGTTTTTCA 50.0 45.0 58.3 56.9 834-849 bp 1.0 70.1
trnK-UUU-rps16_p2 TCTAGCCGCACTTAAAAGCC AGAGGGCAGAGGTTTTTCAA 50.0 45.0 58.3 56.9 833-848 bp 1.0 70.1
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AGGGCAGAGGTTTTTCAAGA 50.0 45.0 58.3 56.9 831-846 bp 1.0 70.1
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AGAGGGCAGAGGTTTTTCAA 50.0 45.0 58.3 56.9 828-843 bp 1.0 70.0
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AGGGCAGAGGTTTTTCAAGA 50.0 45.0 58.3 56.9 826-841 bp 1.0 70.0