Hotspot

ccsA-ndhD

SSC · 116768-116963 · 196 bp

Back to Acer

Pi 0.0467
MarkerSeek score 71.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
196
variable_sites
43
pi
0.0467
unique_haplotype_count
52
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTGGCTTCTATGGGCTTTGT TCCGGATTTCGTTCTGTCAC 50.0 50.0 58.2 57.9 366-380 bp 1.0 87.2
ccsA-ndhD_p2 TGTGGCTTCTATGGGCTTTG TCCGGATTTCGTTCTGTCAC 50.0 50.0 58.2 57.9 367-381 bp 1.0 87.2
ccsA-ndhD_p3 GTGGCTTCTATGGGCTTTGT CCGGATTTCGTTCTGTCACT 50.0 50.0 58.2 57.9 365-379 bp 1.0 87.2
ccsA-ndhD_p4 TGTGGCTTCTATGGGCTTTG CCGGATTTCGTTCTGTCACT 50.0 50.0 58.2 57.9 366-380 bp 1.0 87.1
ccsA-ndhD_p5 TTCCGCAATTGTGGCTTCTA TCCGGATTTCGTTCTGTCAC 45.0 50.0 57.8 57.9 376-390 bp 0.992537 86.6