Hotspot

petA-psbJ

LSC · 64550-65311 · 762 bp

Back to Acer

Pi 0.0156
MarkerSeek score 66.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
762
variable_sites
144
pi
0.0156
unique_haplotype_count
93
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCTGTTGTTTTGGCACA TATTCCGGATTGGGTTCGTC 45.0 50.0 57.8 57.4 741-933 bp 1.0 85.4
petA-psbJ_p2 GCATCTGTTGTTTTGGCACA TCATATTCCGGATTGGGTTCG 45.0 47.6 57.8 57.9 744-936 bp 1.0 81.3
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA ATATTCCGGATTGGGTTCGTC 45.0 47.6 57.8 57.9 742-934 bp 1.0 81.3
petA-psbJ_p4 GCATCTGTTGTTTTGGCACA CATATTCCGGATTGGGTTCGT 45.0 47.6 57.8 58.2 743-935 bp 1.0 81.0
petA-psbJ_p5 GCATCTGTTGTTTTGGCACA TGGTTCATATTCCGGATTGGG 45.0 47.6 57.8 57.8 748-940 bp 0.992537 80.1