Hotspot

trnE-UUC-trnT-GGU

LSC · 31140-31900 · 761 bp

Back to Acer

Pi 0.0176
MarkerSeek score 66.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
761
variable_sites
149
pi
0.0176
unique_haplotype_count
76
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 831-900 bp 1.0 89.7
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 810-879 bp 0.992537 88.9
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 852-921 bp 0.947761 87.8
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 831-900 bp 0.940299 87.0
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 828-897 bp 0.940299 86.8