Hotspot

atpI-rps2

LSC · 15961-16420 · 460 bp

Back to Acer

Pi 0.0168
MarkerSeek score 57.80
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
460
variable_sites
79
pi
0.0168
unique_haplotype_count
87
primer_available
yes
alignment_reliability
0.94
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 TGGGCATGGACTTGTAAACC GGAAATGGAATCCTAGGGGG 50.0 55.0 58.1 57.0 608 bp 0.007463 31.8
atpI-rps2_p2 ATGTTGGCCTACTTCCACAC GGAAATGGAATCCTAGGGGG 50.0 55.0 57.8 57.0 570 bp 0.007463 30.9
atpI-rps2_p3 GTACTTGGGCATGGACTTGT GGAAATGGAATCCTAGGGGG 50.0 55.0 57.8 57.0 613 bp 0.007463 30.9
atpI-rps2_p4 GGGCATGGACTTGTAAACCT GGAAATGGAATCCTAGGGGG 50.0 55.0 57.8 57.0 607 bp 0.007463 30.7
atpI-rps2_p5 AGTACTTGGGCATGGACTTG GGAAATGGAATCCTAGGGGG 50.0 55.0 57.5 57.0 614 bp 0.007463 28.5