Hotspot

psbK-psbI

LSC · 8067-8436 · 370 bp

Back to Acer

Pi 0.0254
MarkerSeek score 63.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
370
variable_sites
86
pi
0.0254
unique_haplotype_count
104
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACAAAGAGTTTGAGAGTAAGCA 50.0 36.4 57.9 55.6 384-449 bp 1.0 56.4
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 381-446 bp 1.0 54.4
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 382-447 bp 1.0 54.4
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 383-448 bp 1.0 54.4
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG AACAAAGAGTTTGAGAGTAAGCA 50.0 34.8 57.9 56.3 385-450 bp 1.0 54.2