Hotspot

trnK-UUU-rps16

LSC · 4378-5307 · 930 bp

Back to Acer

Pi 0.021
MarkerSeek score 64.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
930
variable_sites
242
pi
0.021
unique_haplotype_count
99
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1184-1463 bp 0.977612 90.4
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1180-1459 bp 0.977612 89.9
trnK-UUU-rps16_p3 TTCAGGATCAGTCGTGGTCT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1181-1460 bp 0.977612 89.9
trnK-UUU-rps16_p4 CCCGATGGTATGGACGAATC GCCGTACGAGGAGAAAACTT 55.0 50.0 57.9 57.9 1151-1430 bp 0.850746 84.8
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC GCCGTACGAGGAGAAAACTT 55.0 50.0 57.9 57.9 1150-1429 bp 0.850746 84.8