Hotspot

ycf1

SSC · 120972-126338 · 5367 bp

Back to Acanthus

Pi 0.0524

MarkerSeek score 71.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5367
variable_sites: 411
pi: 0.0524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGTAGCGAAATGGGCGATT	AGTCTGGATACGGCAAAACC	45.0	50.0	58.0	57.9	441-480 bp	1.0	90.0
ycf1_p2	ATGTAGCGAAATGGGCGATT	GGCCCTCCTATCTCTTCCTT	45.0	55.0	58.0	57.9	895-946 bp	1.0	85.9
ycf1_p3	ATGTAGCGAAATGGGCGATT	TCCGAGCTGAGGTTATGGAA	45.0	50.0	58.0	58.1	874-925 bp	1.0	85.9
ycf1_p4	ATGTAGCGAAATGGGCGATT	CCGAGCTGAGGTTATGGAAG	45.0	55.0	58.0	57.8	873-924 bp	1.0	85.1
ycf1_p5	ATGTAGCGAAATGGGCGATT	GTCTGGATACGGCAAAACCA	45.0	50.0	58.0	58.2	440-452 bp	0.666667	75.6