Hotspot

ycf1-rpl32

SSC · 108333-111016 · 2684 bp

Back to Acanthus

Pi 0.0437

MarkerSeek score 73.30

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 2684
variable_sites: 160
pi: 0.0437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	AGGCAGAATACCGTCTTCCA	AATACCCCTTCCCCTTCCAA	50.0	50.0	58.4	57.9	2878 bp	0.333333	49.2
ycf1-rpl32_p2	AGGCAGAATACCGTCTTCCA	ATACCCCTTCCCCTTCCAAA	50.0	50.0	58.4	57.9	2877 bp	0.333333	49.2
ycf1-rpl32_p3	AGGCAGAATACCGTCTTCCA	TACCCCTTCCCCTTCCAAAA	50.0	50.0	58.4	58.2	2876 bp	0.333333	48.7
ycf1-rpl32_p4	GGCAGAATACCGTCTTCCAT	AATACCCCTTCCCCTTCCAA	50.0	50.0	57.4	57.9	2877 bp	0.333333	47.7
ycf1-rpl32_p5	GGCAGAATACCGTCTTCCAT	ATACCCCTTCCCCTTCCAAA	50.0	50.0	57.4	57.9	2876 bp	0.333333	47.7