Pi
0.0603
MarkerSeek score
80.00
Primer available
yes
Label
petA-psbJ
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbJ_p1 | CAGGGCCAGAACTTCTTGTT | CTGGACGGATTCCTCTTTGG | 50.0 | 55.0 | 58.0 | 58.0 | 948-1122 bp | 1.0 | 88.9 |
| petA-psbJ_p2 | TTTATCCCGACGGAAGCAAG | CTGGACGGATTCCTCTTTGG | 50.0 | 55.0 | 58.0 | 58.0 | 1098-1272 bp | 1.0 | 88.7 |
| petA-psbJ_p3 | CAGGGCCAGAACTTCTTGTT | CTTAGCCCATTCGGAAGGAC | 50.0 | 55.0 | 58.0 | 58.0 | 1056-1230 bp | 1.0 | 88.5 |
| petA-psbJ_p4 | CAGGGCCAGAACTTCTTGTT | ACACAATCAAACCCGAACGA | 50.0 | 45.0 | 58.0 | 58.0 | 1208-1382 bp | 1.0 | 88.1 |
| petA-psbJ_p5 | CAGGGCCAGAACTTCTTGTT | GAGCCGACAAGGAATTCCAT | 50.0 | 50.0 | 58.0 | 58.0 | 1447-1621 bp | 1.0 | 87.7 |