Hotspot

rps4-trnT-UGU

LSC · 45858-46321 · 464 bp

Back to Acanthus

Pi 0.0772

MarkerSeek score 84.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 47
pi: 0.0772
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGGCGGCGTATCTTTTTGAA	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.2	57.9	539-770 bp	1.0	87.5
rps4-trnT-UGU_p2	AGGCGGCGTATCTTTTTGAA	ATGGTCATCGGTTCGATTCC	45.0	50.0	58.2	57.8	507-738 bp	1.0	86.9
rps4-trnT-UGU_p3	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	560-791 bp	1.0	85.4
rps4-trnT-UGU_p4	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	528-759 bp	1.0	84.9
rps4-trnT-UGU_p5	AGGCGGCGTATCTTTTTGAA	CCGCTTAGCTCAGAGGTTAG	45.0	55.0	58.2	57.5	547-778 bp	1.0	84.4