Hotspot

trnG-GCC-trnfM-CAU

LSC · 36369-36539 · 171 bp

Back to Acanthus

Pi 0.0549

MarkerSeek score 80.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 14
pi: 0.0549
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	219-240 bp	1.0	88.1
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	220-241 bp	1.0	88.0
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	213-234 bp	1.0	87.6
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	214-235 bp	1.0	87.6
trnG-GCC-trnfM-CAU_p5	AGAAGATGCGGGTTCGATTC	TCACGGGTTCAAATCCTGTC	50.0	50.0	58.1	57.8	212-233 bp	1.0	87.1