Hotspot

trnE-UUC-trnT-GGU

LSC · 30752-31355 · 604 bp

Back to Acanthus

Pi 0.0549
MarkerSeek score 83.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
604
variable_sites
47
pi
0.0549
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 688-864 bp 1.0 87.2
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 685-861 bp 1.0 87.0
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 650-826 bp 1.0 86.6
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 647-823 bp 1.0 86.4
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC ACCATGGCGTTACTCTACCA 55.0 50.0 57.8 58.4 689-865 bp 1.0 84.2