Hotspot

trnC-GCA-petN

LSC · 27890-28770 · 881 bp

Back to Acanthus

Pi 0.0687

MarkerSeek score 84.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 84
pi: 0.0687
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	945-989 bp	1.0	90.3
trnC-GCA-petN_p2	GGTTCCAAATTTGTCGGCTG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	1258-1288 bp	0.666667	72.0
trnC-GCA-petN_p3	CTGGTTCCAAATTTGTCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	1262-1292 bp	0.666667	71.9
trnC-GCA-petN_p4	CTGGTTCCAAATTTGTCGGC	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	1260-1290 bp	0.666667	71.9
trnC-GCA-petN_p5	GGTTCCAAATTTGTCGGCTG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	1260-1290 bp	0.666667	71.9