Hotspot

rpoB-trnC-GCA

LSC · 26770-27818 · 1049 bp

Back to Acanthus

Pi 0.0569

MarkerSeek score 81.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 80
pi: 0.0569
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	ACAAAGCATCAACGGTCTGT	50.0	45.0	58.0	58.0	1720-1817 bp	0.666667	71.7
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CCTCTGTTCTGCTGCTACTG	50.0	55.0	58.0	58.0	1698-1844 bp	0.666667	69.8
rpoB-trnC-GCA_p3	TTGTTTCCTACTCACGCGAG	CCTCTGTTCTGCTGCTACTG	50.0	55.0	57.9	58.0	1714-1860 bp	0.666667	69.5
rpoB-trnC-GCA_p4	GCCTACGCAAGCAAATTGTT	CCTCTGTTCTGCTGCTACTG	45.0	55.0	57.9	58.0	1888-2034 bp	0.666667	69.2
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	TCGCCGAACAGAGGATAGAT	50.0	50.0	58.0	58.0	1671 bp	0.333333	53.0