Hotspot

atpA-atpF

LSC · 11525-11607 · 83 bp

Back to Acanthus

Pi 0.104

MarkerSeek score 80.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 12
pi: 0.104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	AATTTCGTCGGCTCGAATGG	GAGCAATGAAAGAAATAACTGA	50.0	31.8	59.0	52.8	132-171 bp	1.0	48.7
atpA-atpF_p2	ACTAATTTCGTCGGCTCGAA	GAGCAATGAAAGAAATAACTGA	45.0	31.8	57.4	52.8	135-174 bp	1.0	48.6
atpA-atpF_p3	ACTAATTTCGTCGGCTCGAA	GAGCAATGAAAGAAATAACTGAT	45.0	30.4	57.4	53.4	135-174 bp	1.0	48.6
atpA-atpF_p4	ACTAATTTCGTCGGCTCGAAT	GAGCAATGAAAGAAATAACTGA	42.9	31.8	57.8	52.8	135-174 bp	1.0	48.6
atpA-atpF_p5	ACTAATTTCGTCGGCTCGAA	GAGCAATGAAAGAAATAACTGATT	45.0	29.2	57.4	54.2	135-174 bp	1.0	48.6