Hotspot

psbK-psbI

LSC · 7448-7820 · 373 bp

Back to Acanthus

Pi 0.115

MarkerSeek score 78.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 62
pi: 0.115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	ACGAAGAGTTTCAGAGTAAGCA	50.0	40.9	58.0	57.4	419-578 bp	1.0	67.9
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	57.9	57.4	417-576 bp	1.0	67.6
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	58.1	57.4	418-577 bp	1.0	67.3
psbK-psbI_p4	GCCGCTGTAAGTTTTCGATG	ACGAAGAGTTTCAGAGTAAGCA	50.0	40.9	57.8	57.4	416-575 bp	1.0	67.1
psbK-psbI_p5	GCAAGCCGCTGTAAGTTTTC	ACGAAGAGTTTCAGAGTAAGCA	50.0	40.9	58.3	57.4	420-579 bp	1.0	65.8