Hotspot

rpoC1

LSC · 19901-22706 · 2806 bp

Back to Acanthophyllum

Pi 0.00466

MarkerSeek score 59.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2806
variable_sites: 13
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAAAGACTCGAATTCCACGGA	GCTCGTTCGAGAACTACGAT	47.6	50.0	58.0	57.5	2958-2973 bp	1.0	68.8
rpoC1_p2	GAAAGACTCGAATTCCACGGA	CTGCTCGTTCGAGAACTACG	47.6	55.0	58.0	58.5	2960-2975 bp	1.0	68.6
rpoC1_p3	AAAGACTCGAATTCCACGGA	GCTCGTTCGAGAACTACGAT	45.0	50.0	56.9	57.5	2957-2972 bp	1.0	68.1
rpoC1_p4	AAAGACTCGAATTCCACGGA	CTGCTCGTTCGAGAACTACG	45.0	55.0	56.9	58.5	2959-2974 bp	1.0	68.0
rpoC1_p5	GAAAGACTCGAATTCCACGGA	ACTGCTCGTTCGAGAACTAC	47.6	50.0	58.0	57.4	2961-2976 bp	1.0	67.5