Hotspot

psbA

LSC · 274-1335 · 1062 bp

Back to Acanthophyllum

Pi 0.000942

MarkerSeek score 44.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CTACATCCGCCCTTTTGTGT	TTGACACGGGCATATAAGGC	50.0	50.0	58.2	58.0	1386-1387 bp	1.0	78.7
psbA_p2	TACATCCGCCCTTTTGTGTC	TTGACACGGGCATATAAGGC	50.0	50.0	58.2	58.0	1385-1386 bp	1.0	78.7
psbA_p3	CTACATCCGCCCTTTTGTGT	TGGGGAGGGGTTTTTCCTAT	50.0	50.0	58.2	57.9	1591-1595 bp	1.0	78.3
psbA_p4	TACATCCGCCCTTTTGTGTC	TGGGGAGGGGTTTTTCCTAT	50.0	50.0	58.2	57.9	1590-1594 bp	1.0	78.3
psbA_p5	TCACAATCCACTGCCTTGAG	TTGACACGGGCATATAAGGC	50.0	50.0	57.8	58.0	1415 bp	0.5	58.2