Hotspot

trnN-GUU-trnR-ACG

IRa · 127790-128376 · 587 bp

Back to Acanthophyllum

Pi 0.00681

MarkerSeek score 62.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 587
variable_sites: 4
pi: 0.00681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TATCAGATCGAGTCTCGCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	744 bp	1.0	77.6
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	679 bp	1.0	77.5
trnN-GUU-trnR-ACG_p3	CGGTCGGCTGTTAACTGATT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.3	57.8	698 bp	1.0	76.5
trnN-GUU-trnR-ACG_p4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	722 bp	1.0	76.2
trnN-GUU-trnR-ACG_p5	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	671 bp	1.0	76.1