Hotspot

rpl32-trnL-UAG

SSC · 111163-112122 · 960 bp

Back to Acanthophyllum

Pi 0.0115
MarkerSeek score 65.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
960
variable_sites
11
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGCGTTAAAAGGTTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 1096-1114 bp 1.0 78.7
rpl32-trnL-UAG_p2 GTATTGGGCGGCGTTAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 1102-1120 bp 1.0 78.4
rpl32-trnL-UAG_p3 GGCGGCGTTAAAAGGTTTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 1089-1107 bp 1.0 78.2
rpl32-trnL-UAG_p4 GGCGGCGTTAAAAGGTTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 1095-1113 bp 1.0 78.1
rpl32-trnL-UAG_p5 GGCGGCGTTAAAAGGTTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 1094-1112 bp 1.0 78.1