Hotspot

trnR-ACG-trnN-GUU

IRb · 105340-105926 · 587 bp

Back to Acanthophyllum

Pi 0.00681

MarkerSeek score 62.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 587
variable_sites: 4
pi: 0.00681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TATCAGATCGAGTCTCGCGT	55.0	50.0	57.8	58.1	744 bp	1.0	77.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	679 bp	1.0	77.5
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CGGTCGGCTGTTAACTGATT	55.0	50.0	57.8	58.3	698 bp	1.0	76.5
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	722 bp	1.0	76.2
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	55.0	55.0	57.8	57.7	671 bp	1.0	76.1