Hotspot

psaJ-rpl33

LSC · 66095-66549 · 455 bp

Back to Acanthophyllum

Pi 0.0132

MarkerSeek score 63.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 6
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCGGATGCATTGACA	CGGACATCTTTCCCCTTAGC	45.0	55.0	58.1	58.0	518-519 bp	1.0	80.2
psaJ-rpl33_p2	TTCCCGGATGCATTGACATT	CGGACATCTTTCCCCTTAGC	45.0	55.0	57.9	58.0	516-517 bp	1.0	79.7
psaJ-rpl33_p3	TTTCCCGGATGCATTGACAT	CGGACATCTTTCCCCTTAGC	45.0	55.0	57.9	58.0	517-518 bp	1.0	79.7
psaJ-rpl33_p4	TGGTTTGGGTCTTTAGCTGG	CGGACATCTTTCCCCTTAGC	50.0	55.0	57.7	58.0	561-562 bp	1.0	78.5
psaJ-rpl33_p5	GGTTTGGGTCTTTAGCTGGT	CGGACATCTTTCCCCTTAGC	50.0	55.0	57.7	58.0	560-561 bp	1.0	78.5