Hotspot

psbE-petL

LSC · 63486-64761 · 1276 bp

Back to Acanthophyllum

Pi 0.00784

MarkerSeek score 63.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 10
pi: 0.00784
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.9	57.5	1437-1446 bp	1.0	68.4
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	1360-1369 bp	1.0	67.6
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GGAAGTTGTAGTTAAAGCCGC	45.0	47.6	58.2	57.5	1446-1455 bp	1.0	67.4
psbE-petL_p4	TGCTGACGAATAACCAACCC	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	58.2	57.5	1451-1460 bp	1.0	67.3
psbE-petL_p5	CGAATAACCAACCCGCAATG	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	1445-1454 bp	1.0	67.3