Hotspot

ycf4-cemA

LSC · 59002-59773 · 772 bp

Back to Acanthophyllum

Pi 0.00518
MarkerSeek score 61.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
772
variable_sites
4
pi
0.00518
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CTTTGACCCGTACGGATGAA TTGTTTATGAACTCGTCGCA 50.0 40.0 57.6 55.7 891 bp 1.0 59.3
ycf4-cemA_p2 CTTTGACCCGTACGGATGAA TGTTTATGAACTCGTCGCAT 50.0 40.0 57.6 55.5 890 bp 1.0 57.4
ycf4-cemA_p3 CATTCCTTTGACCCGTACGG TTGTTTATGAACTCGTCGCA 55.0 40.0 58.6 55.7 896 bp 1.0 57.2
ycf4-cemA_p4 CCATTCCTTTGACCCGTACG TTGTTTATGAACTCGTCGCA 55.0 40.0 58.6 55.7 897 bp 1.0 57.2
ycf4-cemA_p5 TTCCTTTGACCCGTACGGAT TTGTTTATGAACTCGTCGCA 50.0 40.0 58.7 55.7 894 bp 1.0 56.4