Hotspot

rbcL-accD

LSC · 55559-56304 · 746 bp

Back to Acanthophyllum

Pi 0.00276

MarkerSeek score 63.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 2
pi: 0.00276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCTCGCGAGGGTAATACTA	CCACACCCGTGTTCTAACTC	50.0	55.0	58.0	58.2	903-923 bp	1.0	80.1
rbcL-accD_p2	TCGAGAAGCTTGCAAATGGA	CCACACCCGTGTTCTAACTC	45.0	55.0	57.8	58.2	879-899 bp	1.0	78.9
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	CCACACCCGTGTTCTAACTC	55.0	55.0	57.7	58.2	861-881 bp	1.0	78.1
rbcL-accD_p4	TGCTCGCGAGGGTAATACTA	ATCCACACCCGTGTTCTAAC	50.0	50.0	58.0	57.5	905-925 bp	1.0	78.0
rbcL-accD_p5	AAGCTTGCAAATGGAGTCCT	CCACACCCGTGTTCTAACTC	45.0	55.0	57.7	58.2	874-894 bp	1.0	78.0