Hotspot

psaA-ycf3

LSC · 40712-41795 · 1084 bp

Back to Acanthophyllum

Pi 0.00555

MarkerSeek score 62.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 6
pi: 0.00555
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGTCCGGGTCTGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.1	57.8	1212-1214 bp	1.0	78.3
psaA-ycf3_p2	TGAAAAATGTCCGGGTCTGG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	1286-1288 bp	1.0	77.5
psaA-ycf3_p3	ACGACGTTTTTACGGGATCC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.3	57.8	1180-1182 bp	1.0	76.8
psaA-ycf3_p4	ACGACGTTTTTACGGGATCC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.3	57.7	1254-1256 bp	1.0	76.0
psaA-ycf3_p5	TGAAAAATGTCCGGGTCTGG	CGGAGGCTTGGTTTGATCAA	50.0	50.0	58.1	58.5	1294-1296 bp	1.0	76.0