Hotspot

petN-psbM

LSC · 27595-28685 · 1091 bp

Back to Acanthophyllum

Pi 0.00735

MarkerSeek score 62.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 8
pi: 0.00735
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTATGGGGAAGAAGTGGA	GCTACTGCGCTCTTCATTCT	50.0	50.0	57.8	58.1	1193-1195 bp	1.0	78.2
petN-psbM_p2	ATTGTAAGTCTCGCTTGGGC	GCTACTGCGCTCTTCATTCT	50.0	50.0	58.3	58.1	1251-1253 bp	1.0	78.0
petN-psbM_p3	TCGTATGGGGAAGAAGTGGA	TTGCTACTGCGCTCTTCATT	50.0	45.0	57.8	57.9	1195-1197 bp	1.0	77.9
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	TTGCTACTGCGCTCTTCATT	50.0	45.0	58.3	57.9	1253-1255 bp	1.0	77.8
petN-psbM_p5	CGTATGGGGAAGAAGTGGAC	GCTACTGCGCTCTTCATTCT	55.0	50.0	57.7	58.1	1192-1194 bp	1.0	77.6