Hotspot

atpA-atpF

LSC · 11176-11235 · 60 bp

Back to Acanthophyllum

Pi 0.0333
MarkerSeek score 71.70
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
60
variable_sites
2
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 TTTCGTCTGCTCGAATGGTT AATATTGGCATGTTTGGGGC 45.0 45.0 57.8 57.0 127 bp 1.0 71.9
atpA-atpF_p2 TCGTCTGCTCGAATGGTTAC AATATTGGCATGTTTGGGGC 50.0 45.0 57.7 57.0 125 bp 1.0 71.0
atpA-atpF_p3 ACTTGGAGTACGGTACCAGT AATATTGGCATGTTTGGGGC 50.0 45.0 57.7 57.0 206 bp 1.0 70.3
atpA-atpF_p4 GTCTGCTCGAATGGTTACCA AATATTGGCATGTTTGGGGC 50.0 45.0 57.6 57.0 123 bp 1.0 70.2
atpA-atpF_p5 ATTTCGTCTGCTCGAATGGT AATATTGGCATGTTTGGGGC 45.0 45.0 57.6 57.0 128 bp 1.0 70.1