Hotspot

trnS-GCU-trnG-UCC

LSC · 7794-8533 · 740 bp

Back to Acanthophyllum

Pi 0.00813

MarkerSeek score 63.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 6
pi: 0.00813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	TCAAACCGAGAGACCCCTTA	55.0	50.0	58.1	57.7	905 bp	1.0	77.6
trnS-GCU-trnG-UCC_p2	CACGGAAAGAGAGGGATTCG	AACCGAGAGACCCCTTAACT	55.0	50.0	58.1	57.7	902 bp	1.0	77.6
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	AATCAAACCGAGAGACCCCT	55.0	50.0	58.1	58.3	907 bp	1.0	77.4
trnS-GCU-trnG-UCC_p4	CACGGAAAGAGAGGGATTCG	ATCAAACCGAGAGACCCCTT	55.0	50.0	58.1	58.3	906 bp	1.0	77.4
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TCAAACCGAGAGACCCCTTA	50.0	50.0	58.2	57.7	904 bp	1.0	77.0