Hotspot

rpl16

LSC · 82478-84077 · 1600 bp

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Pi 0.0133
MarkerSeek score 52.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1600
variable_sites
21
pi
0.0133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGGGCACTGCTTCTTTGATT GCAAATTGCAGGACGTATCG 45.0 50.0 58.0 57.9 2201-2211 bp 1.0 80.8
rpl16_p2 TTGGGCACTGCTTCTTTGAT GCAAATTGCAGGACGTATCG 45.0 50.0 58.0 57.9 2202-2212 bp 1.0 80.8
rpl16_p3 ATTGGGCACTGCTTCTTTGA GAACACGGGGAATCGAAGAA 45.0 50.0 58.0 57.9 2426-2436 bp 1.0 80.6
rpl16_p4 TGGGCACTGCTTCTTTGATT GAACACGGGGAATCGAAGAA 45.0 50.0 58.0 57.9 2424-2434 bp 1.0 80.6
rpl16_p5 TTGGGCACTGCTTCTTTGAT GAACACGGGGAATCGAAGAA 45.0 50.0 58.0 57.9 2425-2435 bp 1.0 80.6