Hotspot

ndhG-ndhI

SSC · 115519-115527 · 9 bp

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Pi 0.111
MarkerSeek score 76.80
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
9
variable_sites
1
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 ACTATTCCCAGACCTCCTAA GCCACAAGGTACATTGGACA 45.0 50.0 54.2 58.1 127 bp 1.0 49.4
ndhG-ndhI_p2 ACTATTCCCAGACCTCCTAA TGAGCCACAAGGTACATTGG 45.0 50.0 54.2 57.8 130 bp 1.0 48.6
ndhG-ndhI_p3 ACTATTCCCAGACCTCCTAA AGCCACAAGGTACATTGGAC 45.0 50.0 54.2 57.8 128 bp 1.0 48.6
ndhG-ndhI_p4 ATACTATTCCCAGACCTCCT GCCACAAGGTACATTGGACA 45.0 50.0 53.9 58.1 129 bp 1.0 47.4
ndhG-ndhI_p5 ACTATTCCCAGACCTCCTAAT GCCACAAGGTACATTGGACA 42.9 50.0 54.8 58.1 127 bp 1.0 46.6