Hotspot

rps8-rpl14

LSC · 81757-81984 · 228 bp

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Pi 0.0221
MarkerSeek score 66.00
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
228
variable_sites
5
pi
0.0221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATAATGTCCCTACCC TGGTGCAATTGCTCGAGAAT 55.0 45.0 58.1 58.2 323 bp 1.0 79.5
rps8-rpl14_p2 CGGCAATAATGTCCCTACCC ATTGCTCGAGAATTGCGACA 55.0 45.0 58.1 58.3 316 bp 1.0 78.7
rps8-rpl14_p3 CGGCAATAATGTCCCTACCC TTGGTGCAATTGCTCGAGAA 55.0 45.0 58.1 58.4 324 bp 1.0 77.7
rps8-rpl14_p4 CGGCAATAATGTCCCTACCC TTTGGTGCAATTGCTCGAGA 55.0 45.0 58.1 58.4 325 bp 1.0 77.7
rps8-rpl14_p5 CGGCAATAATGTCCCTACCC TTTTGGTGCAATTGCTCGAG 55.0 45.0 58.1 57.6 326 bp 1.0 77.5