Hotspot

rpoA-rps11

LSC · 80113-80211 · 99 bp

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Pi 0.0404
MarkerSeek score 73.90
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
99
variable_sites
4
pi
0.0404
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 ACACATTTCCACTGCAGTGT AAGAGATGCGGCATTACGAG 45.0 50.0 57.9 58.1 267 bp 1.0 79.6
rpoA-rps11_p2 ACACATTTCCACTGCAGTGT GAAGAGATGCGGCATTACGA 45.0 50.0 57.9 58.1 268 bp 1.0 79.6
rpoA-rps11_p3 ACGCTTGCTGTCTACTCTTG AAGAGATGCGGCATTACGAG 50.0 50.0 57.9 58.1 292 bp 1.0 79.4
rpoA-rps11_p4 ACGCTTGCTGTCTACTCTTG GAAGAGATGCGGCATTACGA 50.0 50.0 57.9 58.1 293 bp 1.0 79.3
rpoA-rps11_p5 GGACAGAACGAAACGACCAT AAGAGATGCGGCATTACGAG 50.0 50.0 58.2 58.1 319 bp 1.0 78.2