Hotspot

clpP-psbB

LSC · 72631-73313 · 683 bp

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Pi 0.0089
MarkerSeek score 63.50
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
683
variable_sites
6
pi
0.0089
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CAACCAGAAACCAGAGCTGT 50.0 50.0 58.0 58.0 848-857 bp 1.0 81.2
clpP-psbB_p2 ACCCAAACTGCATCTTCCTC CAACCAGAAACCAGAGCTGT 50.0 50.0 57.8 58.0 834-843 bp 1.0 79.6
clpP-psbB_p3 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 770-779 bp 1.0 79.6
clpP-psbB_p4 TCCTCTCCAGGATTCCGAAA CAACCAGAAACCAGAGCTGT 50.0 50.0 57.8 58.0 819-828 bp 1.0 79.3
clpP-psbB_p5 TACGTCAACCCAAACTGCAT CAACCAGAAACCAGAGCTGT 45.0 50.0 57.7 58.0 841-850 bp 1.0 79.1