Hotspot

petA-psbJ

LSC · 63345-64285 · 941 bp

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Pi 0.0128
MarkerSeek score 56.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
941
variable_sites
12
pi
0.0128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TCTGTTCTTCTTGGCATCGG TCAGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 1062-1065 bp 1.0 79.8
petA-psbJ_p2 TGGCATCGGTGATTTTAGCA TCAGGGTTGGGTTCATCTCT 45.0 50.0 57.9 58.0 1051-1054 bp 1.0 79.7
petA-psbJ_p3 TCTGTTCTTCTTGGCATCGG CAGGGTTGGGTTCATCTCTG 50.0 55.0 57.9 57.9 1061-1064 bp 1.0 79.2
petA-psbJ_p4 GAGTCCAAGGCCTTCTGTTC TCAGGGTTGGGTTCATCTCT 55.0 50.0 58.2 58.0 1075-1078 bp 1.0 79.1
petA-psbJ_p5 CTGTTCTTCTTGGCATCGGT TCAGGGTTGGGTTCATCTCT 50.0 50.0 58.2 58.0 1061-1064 bp 1.0 79.1