Hotspot

ndhJ-ndhK

LSC · 49579-49704 · 126 bp

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Pi 0.0397
MarkerSeek score 66.80
Primer available yes
Label ndhJ-ndhK

Feature Metadata

label_name
ndhJ-ndhK
feature_type
igs
region
LSC
length_bp
126
variable_sites
5
pi
0.0397
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-ndhK_p1 GGAATTCCCAATCCTCGGG ACCTTCGACACAGTACTCAT 57.9 45.0 57.6 55.9 297 bp 0.5 32.0
ndhJ-ndhK_p2 TGGAATTCCCAATCCTCGGG ACCTTCGACACAGTACTCAT 55.0 45.0 59.4 55.9 298 bp 0.5 31.7
ndhJ-ndhK_p3 ATGACAGCAATGGAATTCCC ACCTTCGACACAGTACTCAT 45.0 45.0 56.1 55.9 308 bp 0.5 27.7
ndhJ-ndhK_p4 GGAATTCCCAATCCTCGGG ACCTTCGACACAGTACTCATAC 57.9 45.5 57.6 57.3 297 bp 0.5 27.0
ndhJ-ndhK_p5 TGGAATTCCCAATCCTCGGG ACCTTCGACACAGTACTCATAC 55.0 45.5 59.4 57.3 298 bp 0.5 26.8