Hotspot

atpH-atpI

LSC · 14284-14932 · 649 bp

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Pi 0.0077
MarkerSeek score 63.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
649
variable_sites
5
pi
0.0077
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CGCAATACCTTCTACGGCTT AACTTTGGCTGCGGCTTATA 50.0 45.0 58.1 57.9 818 bp 1.0 79.0
atpH-atpI_p2 TCCGATAGAAGCAAGCCCTA AACTTTGGCTGCGGCTTATA 50.0 45.0 57.9 57.9 767 bp 1.0 78.9
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA AACTTTGGCTGCGGCTTATA 45.0 45.0 57.9 57.9 746 bp 1.0 78.4
atpH-atpI_p4 CCGATAGAAGCAAGCCCTAC AACTTTGGCTGCGGCTTATA 55.0 45.0 57.8 57.9 766 bp 1.0 78.3
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC AACTTTGGCTGCGGCTTATA 50.0 45.0 57.8 57.9 745 bp 1.0 77.8