Hotspot

matK-trnK-UUU

LSC · 3272-4400 · 1129 bp

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Pi 0.00714
MarkerSeek score 63.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1129
variable_sites
8
pi
0.00714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCTGAAGGAGTAGCGGATA TCCGGGTTGCTAACTCAATG 50.0 50.0 57.0 57.9 1272-1276 bp 1.0 71.8
matK-trnK-UUU_p2 TCCTGAAGGAGTAGCGGATA CGGGTTGCTAACTCAATGGT 50.0 50.0 57.0 58.2 1270-1274 bp 1.0 71.3
matK-trnK-UUU_p3 TCCTGAAGGAGTAGCGGATA GATCCGGGTTGCTAACTCAA 50.0 50.0 57.0 57.6 1274-1278 bp 1.0 69.5
matK-trnK-UUU_p4 TCCTGAAGGAGTAGCGGATA TTGATCCGGGTTGCTAACTC 50.0 50.0 57.0 57.6 1276-1280 bp 1.0 69.5
matK-trnK-UUU_p5 TCCTGAAGGAGTAGCGGATAT TCCGGGTTGCTAACTCAATG 47.6 50.0 57.5 57.9 1272-1276 bp 1.0 68.0