Hotspot

ndhF-rpl32

SSC · 133894-134858 · 965 bp

Back to Acacia

Pi 0.0469
MarkerSeek score 77.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
965
variable_sites
61
pi
0.0469
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGGAATTGGGCAGACTTAG AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 2184-2327 bp 1.0 87.0
ndhF-rpl32_p2 GGGGAATTGGGCAGACTTAG CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 2185-2328 bp 1.0 87.0
ndhF-rpl32_p3 AAATAACAGGAAGGCGCACA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2151-2294 bp 1.0 86.7
ndhF-rpl32_p4 AATAACAGGAAGGCGCACAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2150-2293 bp 1.0 86.7
ndhF-rpl32_p5 ATAACAGGAAGGCGCACAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2149-2292 bp 1.0 86.7