Hotspot

rps3-rps19

LSC · 91333-92062 · 730 bp

Back to Acacia

Pi 0.0549
MarkerSeek score 77.20
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
730
variable_sites
48
pi
0.0549
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCTGAAACCGAGC TGATAAAAGATCTCGTCGTT 50.0 35.0 57.5 52.1 565-792 bp 1.0 49.6
rps3-rps19_p2 GTCTGAAACCGAGCGGATTT TGATAAAAGATCTCGTCGTT 50.0 35.0 58.6 52.1 559-786 bp 1.0 49.6
rps3-rps19_p3 TACCAAGTCTGAAACCGAGC AATGATAAAAGATCTCGTCGT 50.0 33.3 57.5 52.8 567-794 bp 1.0 49.6
rps3-rps19_p4 TACCAAGTCTGAAACCGAGC ATGATAAAAGATCTCGTCGTT 50.0 33.3 57.5 52.8 566-793 bp 1.0 49.6
rps3-rps19_p5 GTCTGAAACCGAGCGGATTT ATGATAAAAGATCTCGTCGTT 50.0 33.3 58.6 52.8 560-787 bp 1.0 49.6