Hotspot

clpP-psbB

LSC · 79432-79939 · 508 bp

Back to Acacia

Pi 0.0687
MarkerSeek score 78.00
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
508
variable_sites
45
pi
0.0687
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 TCGAATCCAAATCGCGATGT ATACTCCAACCACCCCATGA 45.0 50.0 58.1 58.0 1164-1412 bp 1.0 89.6
clpP-psbB_p2 TCGAATCCAAATCGCGATGT TGATACTCCAACCACCCCAT 45.0 50.0 58.1 58.0 1166-1414 bp 1.0 89.6
clpP-psbB_p3 TCGAATCCAAATCGCGATGT ACCAAAACAAGCTACCCCTG 45.0 50.0 58.1 58.0 1381-1629 bp 1.0 88.9
clpP-psbB_p4 TCGAATCCAAATCGCGATGT ACACCCATATTCCAGGACCA 45.0 50.0 58.1 58.0 1430-1678 bp 1.0 88.7
clpP-psbB_p5 TCGAATCCAAATCGCGATGT ATCAAAACCTTCCACACCCC 45.0 50.0 58.1 58.0 1498-1746 bp 1.0 88.6