Hotspot

rpl33-rps18

LSC · 75035-75279 · 245 bp

Back to Acacia

Pi 0.0747
MarkerSeek score 84.30
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
245
variable_sites
25
pi
0.0747
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTGTTGTTGCAAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 381-476 bp 1.0 84.3
rpl33-rps18_p2 TTCTGTCCCTGTTGTTGCAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 382-477 bp 1.0 84.3
rpl33-rps18_p3 ATTCTGTCCCTGTTGTTGCA TCAATTCGATCCCCCGATTG 45.0 50.0 57.6 57.7 383-478 bp 1.0 82.5
rpl33-rps18_p4 TCTGTCCCTGTTGTTGCAAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 355-450 bp 1.0 82.1
rpl33-rps18_p5 TTCTGTCCCTGTTGTTGCAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 356-451 bp 1.0 82.1