Hotspot

accD-psaI

LSC · 65502-66076 · 575 bp

Back to Acacia

Pi 0.0646
MarkerSeek score 81.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
575
variable_sites
49
pi
0.0646
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GTATGTGCTTCTGGAGGAGC CCATTGCAATTGCCGGAAAT 55.0 45.0 58.1 58.0 1034-1053 bp 1.0 88.9
accD-psaI_p2 GTATGTGCTTCTGGAGGAGC TTGTTTTTCGAGACCCCGTT 55.0 45.0 58.1 58.0 1328-1336 bp 1.0 87.9
accD-psaI_p3 GTATGTGCTTCTGGAGGAGC ATACGTTCTGATCGCCAACC 55.0 50.0 58.1 58.1 1293-1301 bp 1.0 87.8
accD-psaI_p4 GTATGTGCTTCTGGAGGAGC GGATAATGGCCCAGCAGAAA 55.0 50.0 58.1 57.9 1352-1360 bp 1.0 87.5
accD-psaI_p5 GTATGTGCTTCTGGAGGAGC AAAGGATAATGGCCCAGCAG 55.0 50.0 58.1 57.9 1355-1363 bp 1.0 87.5