Hotspot

trnT-UGU-trnL-UAA

LSC · 51416-52905 · 1490 bp

Back to Acacia

Pi 0.0398
MarkerSeek score 78.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1490
variable_sites
76
pi
0.0398
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1348-1572 bp 1.0 86.2
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1356-1580 bp 1.0 85.7
trnT-UGU-trnL-UAA_p3 AAATGCGACGCTCTAACCTC ACCAATTTCGCCATATCCCC 50.0 50.0 58.4 58.0 1320-1544 bp 1.0 85.4
trnT-UGU-trnL-UAA_p4 AAATGCGACGCTCTAACCTC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.4 58.1 1328-1552 bp 1.0 84.8
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1362-1586 bp 1.0 84.3