Hotspot

psbZ-trnG-GCC

LSC · 40859-41571 · 713 bp

Back to Acacia

Pi 0.0419
MarkerSeek score 81.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
713
variable_sites
38
pi
0.0419
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 789-868 bp 1.0 85.0
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG ATACATAAATAGGGGGCGCG 55.0 50.0 58.0 57.6 833-912 bp 1.0 84.2
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 792-871 bp 1.0 83.5
psbZ-trnG-GCC_p4 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 785-864 bp 1.0 83.3
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 790-869 bp 1.0 83.1