Hotspot

trnR-UCU-atpA

LSC · 11560-12061 · 502 bp

Back to Acacia

Pi 0.0434
MarkerSeek score 80.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
502
variable_sites
32
pi
0.0434
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CATTCACTCGGGAAGCAGAA 47.6 50.0 57.3 57.9 556-662 bp 1.0 73.9
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC AAGACATTCACTCGGGAAGC 47.6 50.0 57.3 57.9 560-666 bp 1.0 73.8
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GGAAGCAGAAGCCCTTTTGA 47.6 50.0 57.3 58.4 546-652 bp 1.0 71.7
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC ATTCACTCGGGAAGCAGAAG 47.6 50.0 57.3 57.6 555-661 bp 1.0 71.5
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT CATTCACTCGGGAAGCAGAA 47.6 50.0 56.9 57.9 591-697 bp 1.0 70.8