Hotspot

trnK-UUU-rps16

LSC · 4429-5605 · 1177 bp

Back to Acacia

Pi 0.0827
MarkerSeek score 87.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1177
variable_sites
136
pi
0.0827
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG AAGGCGGGTGTTTTTATGGA 50.0 45.0 58.6 57.7 1285-1488 bp 1.0 85.0
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG AGGCGGGTGTTTTTATGGAA 50.0 45.0 58.6 57.7 1284-1487 bp 1.0 85.0
trnK-UUU-rps16_p3 GAGTCGCACTTAAAAGCCGA AAGGCGGGTGTTTTTATGGA 50.0 45.0 58.6 57.7 1286-1489 bp 1.0 85.0
trnK-UUU-rps16_p4 GAGTCGCACTTAAAAGCCGA AGGCGGGTGTTTTTATGGAA 50.0 45.0 58.6 57.7 1285-1488 bp 1.0 85.0
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG GGCGGGTGTTTTTATGGAAC 50.0 50.0 58.6 57.6 1283-1486 bp 1.0 84.6