Hotspot

rpl16

LSC · 85718-87267 · 1550 bp

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Pi 0.00817

MarkerSeek score 53.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1550
variable_sites: 30
pi: 0.00817
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCGGCGATTACTAGCTCCT	ATCTTCTGGTGTCGAGGGAA	50.0	50.0	58.1	58.1	2419-2515 bp	1.0	81.3
rpl16_p2	ATCGGCGATTACTAGCTCCT	TCTTCTGGTGTCGAGGGAAT	50.0	50.0	58.1	58.1	2418-2514 bp	1.0	81.3
rpl16_p3	ATCGGCGATTACTAGCTCCT	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.1	58.1	2409-2505 bp	1.0	81.3
rpl16_p4	TCAAATGGGTTTGGGCTTGA	ATCTTCTGGTGTCGAGGGAA	45.0	50.0	57.9	58.1	2350-2399 bp	0.571429	62.7
rpl16_p5	TCAAATGGGTTTGGGCTTGA	TCTTCTGGTGTCGAGGGAAT	45.0	50.0	57.9	58.1	2349-2398 bp	0.571429	62.7